oli2go

A tool for multiplex oligonucleotide design.

Hendling, Michaela, et al. Oli2go: an automated multiplex oligonucleotide design tool. Nucleic acids research (2018)

Paste sequences here (minimum of 2 sequences in FASTA format):


or upload FASTA file:

Upload FASTA file with at least two sequences!

Sample data:

1. Push "Load sample data" button to fill input fields with sample data.
2. Push "Start job" button to start design
(File contains 5 sequences, approximate time: 30 minutes)


Sample results:

Push "Load sample results" button to get directly to a complete result output.

Load sample results

Thank you!

Your data is now being processed. You can check the status of your submission at:

Probe settings

Application



Probe length

Min bp Max bp

Probe melting temperature

Min °C Max °C

Monovalent cations

mM

Divalent cations

mM

dNTPs

mM

Probe concentration

nM

Maximum number of IUPAC codes

bp

Databases for probe specificity check

Bacteria Fungi
Invertebrates Viruses
Plants Protozoa
Archaea Environmental samples
Patents WGS projects

Primer settings

Product size

Min bp Max bp

Minimum product size difference


Primer length

Min bp Max bp

Primer melting temperature

Min °C Max °C

Monovalent cations

mM

Divalent cations

mM

dNTPs

mM

Primer concentration

nM

Maximum number of IUPAC codes

bp

Dimerisation thresholds

Probe hairpin Tm threshold

°C

Probe hairpin delta G threshold

cal/mol

Primer hairpin Tm threshold

°C

Primer hairpin delta G threshold

cal/mol

Cross dimer Tm threshold

°C

Cross dimer delta G threshold

cal/mol