A tool for multiplex oligonucleotide design.
Hendling, Michaela, et al. Oli2go: an automated multiplex oligonucleotide design tool. Nucleic acids research (2018)
Oli2go is an efficient, user friendly, fully automated multiplex oligonucleotide design tool for non-human DNA, which includes the design of primers and hybridization probes, specificity and cross dimer checks in one run. The software is specialized in the design of high quality probes and primers for bacteria, viruses, fungi, invertebrates, plants, protozoa and archaea. You only have to input your target sequences (minimum 2 sequences in FASTA format), set the input parameters and press the "Start design" button. For more information, please refer to the help section in the top right corner.
The following steps are accomplished by the software:
Note: In theory as many sequences can be used as wanted. However, as the software works highly accurate, the computation time increases with the number of input sequences. For example 20 sequences (~1000 bp each) at once take approximately 2 hours. Therefore, please choose reasonable input parameters, check your input sequences before submission and plan enough time for design (e.g. run the software over night).
Paste sequences here (minimum of 2 sequences in FASTA format):
or upload FASTA file: